p21/CIP1/CDKN1A Antibody (WA-1)

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463]

Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Western Blot: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] -

p66Shc-/- induces premature cell cycle arrest during aging. (A-B) p21 protein expression in lung homogenate at different ages in WT and p66Shc-/- mice. n=4-5. (C-E) p21 protein expression in lung homogenates from WT and p66Shc-/- mice of corresponding ages. n=6. (F-H) Lamin B1 protein expression in lung homogenates from WT and p66Shc-/- mice of corresponding ages. The expression of p21 and lamin B1 is depicted as a ratio relative to beta-actin expression. (I) Senescence associated beta-galactosidase (SA beta-gal) activity in fresh cryo-sections from lungs of WT and p66Shc-/- mice at 3, 12, and 24 months of age (n= 3-4 mice per group). Thirty parenchymal fields were randomly selected for each lung to quantify the number of beta- galactosidase positive cells. (J-L) mRNA expression of (J) IL6, (K) IL-1 beta beta, and (L) TNF alpha in lung homogenates of p66Shc-/- and WT during aging. n=3 mice per group. Data were log-transformed for analysis. (A-B) Tests were performed as general linear hypothesis tests and P-values were adjusted for multiple testing using the "single-step" method. (C-H)P-values are from Student’s. t-tests. (I-L) Multiple tests were performed as general linear hypothesis tests and P-values were adjusted for multiple testing using the "single-step" method. Main effects of the two predictors were tested by F-tests in corresponding linear models without interaction terms. n=3-6 mice per group. The sample size for each group is indicated in the Supplementary Table 2. *p <0.05, **p≤0.01, ***p ≤0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37548932), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] -

Aldehyde 13-13 acts to reduce cellular senescence: (A) OR2H2 expression comparison between non-senescent (Non-S) and senescent (S) cells via qPCR; (B) Immunocytochemical depiction of Ki-67 (green) in senescent cells exposed to aldehyde 13-13 (40 uM). DAPI is shown in red; (C) Immunoblot assessment of P16 and P21 expression in senescent cells treated with aldehyde 13-13; (D) Examination of morphology and expression of senescence-associated beta-galactosidase (SA beta-Gal) in VK2/E6E7 cells. Tests were performed in three or four replicates. Ctrl, control; Ald 13-13, aldehyde 13-13. Data are presented as mean +/- SD. Two-tailed unpaired t-test was applied for multiple or two-group comparisons. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37765029), licensed under a CC-BY license. Not internally tested by Novus Biologicals.