OPA1 Antibody (1E8-1D9) - BSA Free

Simple Western: OPA1 Antibody (1E8-1D9)BSA Free [NBP1-71656]

Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: OPA1 Antibody (1E8-1D9) -

Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.

Western Blot: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] -

Melatonin-induced miR-4516 improves mitochondrial dynamics and enhances PINK1/Parkin-mediated mitophagy in the CKD mouse model. (A) Representative TEM images of mitochondria in renal cortex of CKD mice either treated with melatonin (0.2 mg/kg), or both melatonin and miR-4516 inhibitor (300 nM). Each group received two intraperitoneal injections per week (every 3–4 days)—a total of 4 injections for 2 weeks. All comparisons were made against healthy kidney control (scare bar = 1 μm) (B,C) Measurement of mitochondrial area and number of abnormal mitochondria in renal cortex of each groups (n = 3). (D) The expression of p-DRP1, DRP1, MFN1, and OPA1 in renal cortex of each group. Protein expression level were quantified by densitometry and normalized to DRP1 or alpha-tubulin levels (n = 3). (E) Immunofluorescence staining for LAMP-1 (green) and COX4 (red) in renal cortex of each group. Scare bar = 20 μm. (F) Expression of LC3B-II/LC3B-I ratio and P62 in renal cortex of each groups (n = 3). The values represent mean +/- SEM, * p < 0.05, ** p < 0.01 versus healthy kidney cortex; #p < 0.05, ##p < 0.01 versus PBS; $p < 0.05, $$p < 0.01 versus melatonin. The alpha-tubulin was used as Western blot loading control for whole tissue lysates. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34359852), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] -

Melatonin-induced miR-4516 rescues abnormal mitochondrial functions. (A) Representative TEM images for TH1 cells either treated with p-Cresol alone, melatonin under p-Cresol exposure, or miR-4516 inhibitor (50 nM for 48 h) before melatonin treatment, compared with TH1 control (scare bar = 1 μm). (B,C) Measurement of mitochondrial area and number of abnormal mitochondria in each experimental group (n = 3). (D) The effects of melatonin on p-DRP1, DRP1, MFN1, and OPA1 were reversed with miR-4516 inhibitor. Protein expression level was detected using western blot, quantified by densitometry, and normalized to DRP1 or VDAC1 levels (n = 3) respectively. (E,F) Measurement of TMRE (E) and MitoSOX (F) positive cells for each group (n = 3). The values represent mean +/- SEM, * p < 0.05, ** p < 0.01 versus control; #p < 0.05, ##p < 0.01 versus p-Cresol exposure; $$p < 0.01 versus melatonin-treated cells in p-Cresol exposure. The beta-actin or VDAC1 was used as Western blot loading control for whole cell lysates or mitochondrial fraction, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34359852), licensed under a CC-BY license. Not internally tested by Novus Biologicals.