OGG1 Antibody (2B4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52724
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0004.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0005.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
![Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Immunocytochemistry-Immunofluorescence-NBP2-52724-img0002.jpg "Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry, IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 2B4
Format
BSA Free
Concentration
0.9 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to an internal sequence of the human OGG1 protein (between amino acids 100-300)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for OGG1 Antibody (2B4) - BSA Free
Immunocytochemistry/ Immunofluorescence: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]
Immunocytochemistry/Immunofluorescence: OGG1 Antibody (2B4) [NBP2-52724] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (2B4) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Immunohistochemistry-Paraffin-NBP2-52724-img0006.jpg "Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) - BSA Free [NBP2-52724]
Immunohistochemistry-Paraffin: OGG1 Antibody (2B4) [NBP2-52724] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells renal cell carcinoma.![Flow (Intracellular): OGG1 Antibody (2B4) - BSA Free [NBP2-52724]](https://resources.bio-techne.com/images/products/OGG1-Antibody-2B4-Flow-Intracellular-NBP2-52724-img0001.jpg "Flow (Intracellular): OGG1 Antibody (2B4) - BSA Free [NBP2-52724]")
Flow (Intracellular): OGG1 Antibody (2B4) - BSA Free [NBP2-52724]
Flow (Intracellular): OGG1 Antibody (2B4) [NBP2-52724] - An intracellular stain was performed on HeLa cells with OGG1 (2B4) antibody NBP2-52724 (Blue) and a matched isotype control NBP2-27231 (Orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).Applications for OGG1 Antibody (2B4) - BSA Free
Application
Recommended Usage
Flow Cytometry
2-5 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.9 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: OGG1
Long Name
8-oxoGuanine DNA Glycosylase
Alternate Names
8-hydroxyguanine DNA glycosylase, 8-oxoguanine DNA glycosylase, AP lyase, DNA-apurinic or apyrimidinic site lyase, HOGG1, MMH, MUTMOGH1HMMH, N-glycosylase/DNA lyase
Gene Symbol
OGG1
Additional OGG1 Products
Product Documents for OGG1 Antibody (2B4) - BSA Free
Product Specific Notices for OGG1 Antibody (2B4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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