NSFL1C Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36529
![Western Blot: NSFL1C Antibody [NBP2-36529]](https://resources.bio-techne.com/images/products/NSFL1C-Antibody-Western-Blot-NBP2-36529-img0001.jpg "Western Blot: NSFL1C Antibody [NBP2-36529]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 25 to 75 of human NSFL1 (p97) Cofactor (p47) using the numbering given in entry NP_057227.2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit NSFL1C Antibody - BSA Free (NBP2-36529) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NSFL1C Antibody - BSA Free
Western Blot: NSFL1C Antibody [NBP2-36529]
Western Blot: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: NSFL1C Antibody [NBP2-36529]](https://resources.bio-techne.com/images/products/NSFL1C-Antibody-Immunoprecipitation-NBP2-36529-img0002.jpg "Immunoprecipitation: NSFL1C Antibody [NBP2-36529]")
Immunoprecipitation: NSFL1C Antibody [NBP2-36529]
Immunoprecipitation: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated NSFL1C, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for NSFL1C Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NSFL1C
Alternate Names
dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, p47, p97 cofactor p47, SHP1 homolog, UBX domain protein 2C, UBX domain-containing protein 2C, UBX1, UBXD10, UBXN2CMGC3347
Gene Symbol
NSFL1C
UniProt
Additional NSFL1C Products
Product Documents for NSFL1C Antibody - BSA Free
Product Specific Notices for NSFL1C Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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