NPM1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90047
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSSTPRS
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%). Human reactivity reported in scientific literature (PMID: 21738817).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NPM1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody [NBP1-90047]
Immunocytochemistry/Immunofluorescence: NPM1 Antibody [NBP1-90047] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.Western Blot: NPM1 Antibody - BSA Free [NBP1-90047]
Analysis in human cell line K562.Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047]
Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.Applications for NPM1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NPM1
NMP antibodies are useful tools as tumor markers in cancer research.
Long Name
Nucleophosmin/Nucleolar Phosphoprotein B23
Alternate Names
B23, Nucleophosmin, Numatrin
Gene Symbol
NPM1
Additional NPM1 Products
Product Documents for NPM1 Antibody - BSA Free
Product Specific Notices for NPM1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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