NOX1 Antibody - Azide Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31546
Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IHC-P WB
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (85%).
Localization
Membrane, Multi-pass membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit NOX1 Antibody - Azide Free (NBP1-31546) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-NOX1 Antibody: Cited in 34 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NOX1 Antibody - Azide Free
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546]
Immunohistochemistry-Paraffin: NOX1 Antibody [NBP1-31546] - Mouse kidney. NOX1 stained by NOX1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Flow Cytometry: NOX1 Antibody [NBP1-31546]
Flow Cytometry: NOX1 Antibody [NBP1-31546] - Nox-1 is present at the surface of resting platelets. Human platelets (4 x 10^8 cells/mL) were isolated from whole blood and incubated with the Nox-1 antibody or Rabbit IgG control at 1:250 dilution for 30 minutes at room temperature. FITC-tagged anti-rabbit (secondary antibody) was added at 1:100 dilution and incubated for an additional 30 minutes. Platelets were then diluted 1:25 v/v in Tyrodes buffer and read using a BD Accuri flow cytometer. Image from verified customer review.Applications for NOX1 Antibody - Azide Free
Application
Recommended Usage
Flow Cytometry
Validated from a verified customer review.
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
Reported in scientific literature (PMID: 27094494)
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP1-31546 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Format
Azide Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NOX1
Long Name
NADPH Oxidase 1
Alternate Names
MOX-1, NOH1
Entrez Gene IDs
27305 (Human)
Gene Symbol
NOX1
UniProt
Additional NOX1 Products
Product Specific Notices for NOX1 Antibody - Azide Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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