Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43558
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Virus
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A1227
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was isolated from an anti-NoV GII.4c IgG serum antibody repertoire generated from three donors that experienced a significant increase in GII.4 titer after immunization with the bivalent GII.4c + GI.1 VLP vaccine. (Uniprot# Q83883)
Specificity
This antibody shows broadly binding but no blocking activity toward multiple GI and GII strains such as GI.I, GII.2, and GII.17. This antibody recognizes a highly conserved epitope located at an occluded site on the norovirus capsid, at the interface between the P domain and shell domain of the Norovirus, which explain its high level of cross-reactivity. Norovirus (NoVs) is one of the main pathogens causing sporadic cases and large outbreaks of acute gastroenteritis, and norovirus can infect people of all ages.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.
Scientific Data Images for Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free
ELISA: Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free [NBP3-43558] -
ELISA: Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free [NBP3-43558] - Binding curves of Norovirus VP1 (red) and an isotype control (anti-fluorescein; orange) on an ELISA plate coated with Norovirus VP1 at 2.5 µg/mL. A 3-fold serial dilution from 3,000 ng/mL to 0.0169 ng/mL was assessed. Detection was performed with an HRP-conjugated anti-rabbit IgG antibody (Goat Anti-Rabbit IgG, Fc-fragment specific, minimum cross-reactivity to human serum proteins) at 0.25 µg/mL.Applications for Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Norovirus VP1
Alternate Names
Capsid Protein, Capsid protein VP1, CP, Norovirus Capsid protein VP1, Norwalk virus VP1, NoV VP1, p59
Additional Norovirus VP1 Products
Product Documents for Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free
Product Specific Notices for Norovirus VP1 Antibody (A1227) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...