NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-115

Novus Biologicals, part of Bio-Techne
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NB300-115
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NMDA R, NR1 Subunit Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C1 splice variant insert conjugated to KLH. Accession # P35439

Reactivity Notes

It is anticipated that the antibody will also work with bovine, canine, and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor containing the C1 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C1 insert.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The antigen is a peptide from the NR1 subunit, C1 splice variant insert of the NMDA receptor. Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Western Blot: NMDA R, NR1 Subunit AntibodyAzide and BSA Free [NB300-115]

Western Blot: NMDA R, NR1 Subunit AntibodyAzide and BSA Free [NB300-115]

Western Blot: NMDA receptor C1 Antibody [NB300-115] - 10 ug of HEK 293 cells expressing: Lane 1 - HEK cells without NR1 expression (Mock); Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the C1 splice variant insert.

Applications for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000-1:2000

Immunohistochemistry-Frozen

1:1000-1:2000

Western Blot

1:1000
Application Notes
NB 300-115 can be used in Western blot where a band is seen at ~ 100 kDa representing the NMDAR1 C1 splice variant.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute with 50 uL PBS to desired concentration.

Formulation

Lyophilized

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDA R, NR1 Subunit

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory and neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2 prime). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 1

Alternate Names

GluN1, MRD8, NDHMSD, NDHMSR;NMD-R1, NMDA R, NR1 Subunit, NMDA1, NMDAR1, NMD-R1, NR1

Gene Symbol

GRIN1

UniProt

Q05586 (Human), P35439 (Rat)

Additional NMDA R, NR1 Subunit Products

Product Documents for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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