I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

Name: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)
Brand: Novus Biologicals
SKU: NBP3-15260

A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15260

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1C2H2 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Nicotinic Acetylcholine R alpha 4/CHRNA4 (P43681). PEADGQAAGALASRNTHSAELPPPDQPSPCKCTCKKEPSSVSPSATVKTRSTKAPPPHLPLSPALTRAVEGVQYIADHLKAEDTDFSVKEDWKYVAMVIDR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2) (NBP3-15260) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2) [NBP3-15260]

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2) [NBP3-15260] - Western blot analysis of extracts of various cell lines, using Nicotinic Acetylcholine R alpha 4/CHRNA4 Rabbit mAb (NBP3-15260) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

Application

Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 4/CHRNA4

Nicotinic Acetylcholine Receptor alpha 4 encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. [provided by RefSeq]

Long Name

Nicotinic Acetylcholine Receptor alpha 4

Alternate Names

BFNC, CHRNA4, EBN1, ENFL1, nAChR alpha 4, nAchRa4, NACRA4, Nicotinic Acetylcholine Ra4

Gene Symbol

CHRNA4

Additional Nicotinic Acetylcholine R alpha 4/CHRNA4 Products

Product Documents for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15260

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Description

Scientific Data Images for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2) [NBP3-15260]

Applications for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: Nicotinic Acetylcholine R alpha 4/CHRNA4

Long Name

Alternate Names

Gene Symbol

Additional Nicotinic Acetylcholine R alpha 4/CHRNA4 Products

Product Documents for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (1C2H2)

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