NFkB1/NFkB p105 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05867
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human NFkB1/NFkB p105.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for NFkB1/NFkB p105 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: NFkB1/NFkB p105 Antibody - BSA Free [NBP3-05867] -
Immunocytochemistry/ Immunofluorescence: NFkB1/NFkB p105 Antibody - BSA Free [NBP3-05867] - Immunofluorescence staining of NFkB1/NFkB p105 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-05867 (dilution ratio 1:1000) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .
Western Blot: NFkB1/NFkB p105 Antibody - BSA Free [NBP3-05867] -
Western Blot: NFkB1/NFkB p105 Antibody - BSA Free [NBP3-05867] - NBP3-05867 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:105 kDa Observed band size:120 kDaApplications for NFkB1/NFkB p105 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NFkB1
Long Name
Nuclear Factor Kappa-B, Subunit 1
Alternate Names
NF-kB1
Gene Symbol
NFKB1
Additional NFkB1 Products
Product Documents for NFkB1/NFkB p105 Antibody - BSA Free
Product Specific Notices for NFkB1/NFkB p105 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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