NDUFS3 Antibody (HL2097)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48671
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2097
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human NDUFS3
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit NDUFS3 Antibody (HL2097) (NBP3-48671) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NDUFS3 Antibody (HL2097)
Western Blot: NDUFS3 Antibody (HL2097) [NBP3-48671] -
Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: NDUFS3 Antibody (HL2097) [NBP3-48671] -
NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: NDUFS3 Antibody (HL2097) [NBP3-48671] -
NDUFS3 antibody [HL2097] detects NDUFS3 protein at mitochondria by immunofluorescent analysis.Sample: 293T cells were fixed in ice-cold MeOH for 5 min.Green: NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .
Applications for NDUFS3 Antibody (HL2097)
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NDUFS3
Alternate Names
CI-30, CI-30kD, complex I 30kDa subunit, Complex I-30kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, NADH dehydrogenase (ubiquinone) Fe-S protein 3 (30kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Qreductase), NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, NADH dehydrogenase-ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit
Gene Symbol
NDUFS3
Additional NDUFS3 Products
Product Documents for NDUFS3 Antibody (HL2097)
Product Specific Notices for NDUFS3 Antibody (HL2097)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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