NARFL Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83611
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KLEASRPDFFNQEHQTRDVDCVLTTGEVFRLLEEEGVSLPDLEPAPLDSLCSGASAEEPTSHRGGGSGGYLEHVFRHAARELFGIHVAEVTYKP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (87%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit NARFL Antibody - BSA Free (NBP1-83611) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-NARFL Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for NARFL Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: NARFL Antibody [NBP1-83611]](https://resources.bio-techne.com/images/products/NARFL-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83611-img0007.jpg "Immunocytochemistry/ Immunofluorescence: NARFL Antibody [NBP1-83611]")
Immunocytochemistry/ Immunofluorescence: NARFL Antibody [NBP1-83611]
Immunocytochemistry/Immunofluorescence: NARFL Antibody [NBP1-83611] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.![Immunohistochemistry-Paraffin: NARFL Antibody [NBP1-83611]](https://resources.bio-techne.com/images/products/NARFL-Antibody-Immunohistochemistry-Paraffin-NBP1-83611-img0002.jpg "Immunohistochemistry-Paraffin: NARFL Antibody [NBP1-83611]")
Immunohistochemistry-Paraffin: NARFL Antibody [NBP1-83611]
Immunohistochemistry-Paraffin: NARFL Antibody [NBP1-83611] - Staining of human kidney shows strong cytoplasmic positivity in distal tubules.![NARFL Antibody - BSA Free Western Blot: NARFL Antibody - BSA Free [NBP1-83611]](https://resources.bio-techne.com/images/products/nbp1-83611_rabbit-polyclonal-narfl-antibody-84202517195677.jpg "Western Blot: NARFL Antibody - BSA Free [NBP1-83611]")
Western Blot: NARFL Antibody - BSA Free [NBP1-83611]
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Applications for NARFL Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NARFL
Alternate Names
cytosolic Fe-S cluster assembly factor NARFL, FLJ21988, HPRN, IOP1Protein related to Narf, Iron-only hydrogenase-like protein 1, LET1 like/JFP15, nuclear prelamin A recognition factor-like, Nuclear prelamin A recognition factor-like protein, PRNLET1L
Gene Symbol
CIAO3
Additional NARFL Products
Product Documents for NARFL Antibody - BSA Free
Product Specific Notices for NARFL Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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