NAB1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86163
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MIGHIFEMNDDDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQISREVTYKYTYRTTKSKCGERDELSPKRIKVE
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NAB1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: NAB1 Antibody [NBP1-86163]
Immunocytochemistry/Immunofluorescence: NAB1 Antibody [NBP1-86163] - Staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & the Golgi apparatus. Antibody staining is shown in green.Western Blot: NAB1 Antibody - BSA Free [NBP1-86163]
Analysis in control (vector only transfected HEK293T lysate) and NAB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: NAB1 Antibody - BSA Free [NBP1-86163]
Staining of human lung shows strong membranous positivity in pneumocytes.Applications for NAB1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NAB1
Alternate Names
EGR1 binding protein 1, EGR-1-binding protein 1, NGFI-A binding protein 1 (EGR1 binding protein 1), NGFI-A-binding protein 1, Transcriptional regulatory protein p54
Gene Symbol
NAB1
Additional NAB1 Products
Product Documents for NAB1 Antibody - BSA Free
Product Specific Notices for NAB1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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