MT-CO2 Antibody (6H0N5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16283
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6H0N5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 128-227 of human MT-CO2 (P00403). LEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVLHSWAVPTLGLKTDAIPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKIFEMGPVFTL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit MT-CO2 Antibody (6H0N5) (NBP3-16283) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MT-CO2 Antibody (6H0N5)
![Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283]](https://resources.bio-techne.com/images/products/MT-CO2-Antibody-6H0N5-Western-Blot-NBP3-16283-img0002.jpg "Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283]")
Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283]
Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Western blot analysis of extracts of various cell lines, using MT-CO2 Rabbit mAb (NBP3-16283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry-Paraffin: MT-CO2 Antibody (6H0N5) [NBP3-16283] -
Immunohistochemistry-Paraffin: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Human liver tissue using MTCO2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry- MT-CO2 Antibody (6H0N5) [NBP3-16283] -
Immunohistochemistry- MT-CO2 Antibody (6H0N5) [NBP3-16283] -Confocal imaging of HeLa cells using MTCO2 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Applications for MT-CO2 Antibody (6H0N5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunocytochemistry/ Immunofluorescence
1:200 - 1:800
Immunohistochemistry
1:200 - 1:800
Immunohistochemistry-Paraffin
1:200 - 1:800
Western Blot
1:1000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MT-CO2
Alternate Names
COII, COX2, COXII, Cytochrome C Oxidase II, Cytochrome C Oxidase Polypeptide II, Cytochrome C Oxidase Subunit II, EC 1.9.3.1, Mitochondrially Encoded Cytochrome C Oxidase II, MTCO2
Gene Symbol
COX2
Additional MT-CO2 Products
Product Documents for MT-CO2 Antibody (6H0N5)
Product Specific Notices for MT-CO2 Antibody (6H0N5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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