MST1/2 [p Thr180, p Thr183] Antibody (JE46-64)

Western Blot: MST1/2 [p Thr180, p Thr183] Antibody (JE46-64) [NBP3-33136]

Western blot analysis on different lysates with NBP3-33136 at 1/1,000 dilution. Lane 1: Ramos cell lysate Lane 2: Ramos treated with 1μM Staurosporine for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33136 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western Blot: MST1/2 [p Thr180, p Thr183] Antibody (JE46-64) [NBP3-33136]

Western blot analysis on different lysates with NBP3-33136 at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate Lane 3: C6 cell lysate Lane 4: C6 treated with 1μM Staurosporine for 3 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33136 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.