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MRP2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-69023

Novus Biologicals, part of Bio-Techne
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NBP1-69023

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to Abcc2 (ATP-binding cassette, sub-family C (CFTR/MRP), member 2) The peptide sequence was selected from the middle region of Abcc2 (NP_038834). Peptide sequence TIQDVNLDIKPGQLVAVVGTVGSGKSSLISAMLGEMENVHGHITIKGSIA. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

174 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for MRP2 Antibody - BSA Free

Western Blot: MRP2 Antibody [NBP1-69023]

Western Blot: MRP2 Antibody [NBP1-69023]

Western Blot: MRP2 Antibody [NBP1-69023] - Sample Tissue: Mouse Thymus Antibody Dilution: 1.0ug/ml
Western Blot: MRP2 Antibody [NBP1-69023]

Western Blot: MRP2 Antibody [NBP1-69023]

Western Blot: MRP2 Antibody [NBP1-69023] - Mouse Spleen lysate, concentration 0.2-1 ug/ml.
MRP2 Antibody - BSA Free

Western Blot: MRP2 Antibody - BSA Free [NBP1-69023] -

a) Relative fold change of ABCC1, ABCC2, ABCG2, and GST in temozolomide (TMZ)- and etoposide (EP)-treated resistant U87-R and non-resistant U87 cells as determined by quantitative real-time PCR. All data were normalized to beta-actin and GAPDH are given relative to control (control=1, not shown in figure). Data are represented as mean +/- SE, n=8. *indicates significantly different values in U87-R vs U87 groups, Mann-Whitney U test: *p ≤0.05; ** p ≤0.01. b) Western blot analysis of ABCC1, ABCC2, ABCG2, and GST proteins (relative density normalized to beta-actin). Data are represented as mean +/- SE, n=3. Control: vehicle-treated control; TMZ: temozolomide, IC50: 250.2 μM; EP: etoposide, IC50: 6.49 μM Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36660193), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MRP2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRP2

Abcc2 mediates hepatobiliary excretion of numerous organic anions. Abcc2 may function as a cellular cisplatin transporter.

Long Name

Multi-drug Resistance Protein 2

Alternate Names

ABC30, ABCC2, CMOAT, CMOAT1, cMRP

Entrez Gene IDs

1244 (Human); 12780 (Mouse)

Gene Symbol

ABCC2

UniProt

Additional MRP2 Products

Product Documents for MRP2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MRP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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