MRGX2 Antibody (477533) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB4727CL1
![MRGX2 Antibody (477533) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab4727cl1_mouse-mrgx2-mab-477533-corafluor-1-311220251357421.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Intracellular Staining by Flow Cytometry, CyTOF-ready
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2A Clone # 477533
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human MRGX2
Met1-Val330
Accession # Q96LB1
Met1-Val330
Accession # Q96LB1
Specificity
Detects human MRGX2. Stains human MRGX2 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for MRGX2 Antibody (477533) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for MRGX2 Antibody (477533) [CoraFluor™ 1]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: MRGX2
MRGX2 (MAS-related GPR, member X2) is G-protein coupled 7-transmembrane protein that is selectively expressed in small-diameter sensory neurons of dorsal root ganglia. Human MRGX shares 41-52% amino acid identity with three other primate MRGX proteins, but has no ortholog in rodents. Binding of ligands such as cortistatin, proadrenaomedullin peptides (PAMP-12 and -20) and basic peptides (substance P, neuropeptide Y) to MRGX2 can activate Gq or Gi regulated pathways. MRGX2 is thought to influence nociception and promote adrenal gland catecholamine secretion and IgE-independent mast cell degranulation.
Long Name
Mas-related G Protein-coupled Receptor Member X2
Alternate Names
MRGPRX2
Gene Symbol
MRGPRX2
Additional MRGX2 Products
Product Documents for MRGX2 Antibody (477533) [CoraFluor™ 1]
Product Specific Notices for MRGX2 Antibody (477533) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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