Mouse TIMP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB980
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Western Blot, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 151620
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1
Cys25-Arg205
Accession # P12032
Cys25-Arg205
Accession # P12032
Specificity
Detects mouse TIMP-1 in ELISAs. In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) TIMP‑1, rhTIMP‑2, rhTIMP-3, rhTIMP-4, or rrTIMP-1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse TIMP-1 Antibody
Mouse TIMP-1 ELISA Standard Curve
Recombinant Mouse TIMP-1 (Catalog # 980-MT) was serially diluted and captured by Rat Anti-Mouse TIMP-1 Monoclonal Antibody (Catalog # MAB980) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF980) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse TIMP-1 Antibody
Mouse TIMP-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-1
Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, proMMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C‑terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N‑terminal domain, TIMP‑1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP-1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.
References
- Murphy, G. and F. Willenbrock (1995) Methods Enzymol. 248:496.
- Brew, K. et al. (2000) Biochim. Biophys. Acta 1477:267.
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
UniProt
Additional TIMP-1 Products
Product Documents for Mouse TIMP-1 Antibody
Product Specific Notices for Mouse TIMP-1 Antibody
For research use only
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