T-bet/TBX21 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse T-bet/TBX21 Monoclonal Antibody (Catalog # MAB11627) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Mouse T-bet/TBX21 by Western Blot.

Western Blot shows lysates of HT-2 mouse T cell line. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse T-bet/TBX21 Monoclonal Antibody (Catalog # MAB11627) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for T-bet/TBX21 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Mouse T-bet/TBX21 Antibody

Application

Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen

Western Blot

2 µg/mL
Sample: HT-2 mouse T cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: T-bet/TBX21

T-box transcription factor TBX21, also known as T-bet, is a 58kDa T-box gene which encodes transcription factors involved in the regulation of developmental processes. TBX21 is a Th1 cell-specific transcription that controls the expression of interferon-gamma. TBX21 also has an important role in many cells of the adaptive and innate immune system and is also expressed in dendritic cells, natural killer cells, natural killer T cells and innate lymphoid cells. T-bet contributes to the maintenance of mucosal homeostasis and mucosal immune response. Mice lacking adaptive immune cells and T-bet developed disease similar to human ulcerative colitis.

References

Lazarevic V, Glimcher LH, Lord GM. T-bet: a bridge between innate and adaptive immunity. Nat Rev Immunol. 2013 Nov;13(11):777-89. doi: 10.1038/nri3536. Epub 2013 Oct 11. PMID: 24113868; PMCID: PMC6290922.
Mohamed R, Lord GM. T-bet as a key regulator of mucosal immunity. Immunology. 2016 Apr;147(4):367-76. doi: 10.1111/imm.12575. Epub 2016 Feb 23. PMID: 26726991; PMCID: PMC4799884.

Long Name

T-box transcription factor TBX21

Alternate Names

T-PET, Tbet, TBLYM, TBX21

Entrez Gene IDs

30009 (Human); 57765 (Mouse); 303496 (Rat)

Gene Symbol

TBX21

UniProt

Q9JKD8 (Rabbit)

Additional T-bet/TBX21 Products

Product Documents for Mouse T-bet/TBX21 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Mouse T-bet/TBX21 Antibody

For research use only

Citations
Reviews

Mouse T-bet/TBX21 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11627

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all T-bet/TBX21 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse T-bet/TBX21 Antibody

Detection of T-bet/TBX21 in Mouse Spleen.