Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3335
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sulfatase Modifying Factor 1/SUMF1
Ser32-Asp372
Accession # Q8R0F3
Ser32-Asp372
Accession # Q8R0F3
Specificity
Detects mouse Sulfatase Modifying Factor 1/SUMF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human SUMF1 is observed and 10% cross-reactivity with recombinant mouse SUMF2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Sulfatase Modifying Factor 1/SUMF1
Sample: Recombinant Mouse Sulfatase Modifying Factor 1/SUMF1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sulfatase Modifying Factor 1/SUMF1
Mammalian sulfatases have a common CXPXR motif, in which the Cys residue is post-translationally modified to a Calpha-formylglycine (FGly) residue. This modification is essential for catalytic activity of all sulfatases and is catalyzed by FGly generating enzyme (FGE) encoded by sulfatase modifying factor 1 (SUMF1). FGE deficiency causes multiple sulfatase deficiency (MSD), a rare autosomal recessive disorder. SUMF2 does not have FGly enzymatic activity, but may compete with SUMF1 for the same substrate.
Alternate Names
FGE1
Gene Symbol
SUMF1
UniProt
Additional Sulfatase Modifying Factor 1/SUMF1 Products
Product Documents for Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody
Product Specific Notices for Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody
For research use only
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