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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SLC39A4
Met1-Tyr337
Accession # Q78IQ7

Specificity

Detects mouse SLC39A4 in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse SLC39A4 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SLC39A4

SLC39A4 (Solute carrier family 39 member 4; also Zip-4) is a 75-90 kDa member of the ZIP transporter family of proteins. It is expressed by select cell types, including pancreatic beta-cells plus epithelium of the choroid plexus and intestine. SLC39A4 is part of a zinc-sensitive cell cycling system. When extracellular zinc levels are high, SLC39A4 is generally found internally associated with endosomes, and is unavailable at the cell membrane to transport zinc into the cell. Under low zinc conditions, SLC39A4 generally appears in the plasma membrane to ensure adequate zinc uptake in the face of limited zinc availability. Mouse SLC39A4 is a 660 amino acid (aa) six transmembrane (TM) glycoprotein. It contains a long N-terminal extracellular region (aa 1-337) followed by six TM segments (aa 338-651) and a short C-terminal nine aa extracellular tail (aa 652-660). There is one splice variant that shows a 16 aa substitution for aa 1-63. SLC39A4 undergoes cell-specific proteolytic processing in response to low zinc concentrations. Cleavage occurs in the N-terminal extracellular region to generate a soluble 35 kDa polypeptide and a 37 kDa six TM integral membrane protein. This does not appear to be an inactivating mechanism. Over aa 1-337, mouse SLC39A4 shares 84% and 59% aa sequence identity with rat and human SLC39A4, respectively.

Long Name

Solute Carrier Family 39 Member 4

Alternate Names

AEZ, ZIP4

Entrez Gene IDs

55630 (Human); 72027 (Mouse); 300051 (Rat)

Gene Symbol

SLC39A4

UniProt

Additional SLC39A4 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse SLC39A4 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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