This antibody detects mouse Reg3D in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Reg1A, 1B, recombinant mouse (rm) Reg1, 2, 3A, 3B, 3G, 4, or recombinant rat Reg3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse Reg3D Antibody

Detection of Reg3D in Mouse Pancreas.

Reg3D was detected in immersion fixed paraffin-embedded sections of mouse pancreas using Rat Anti-Mouse Reg3D Monoclonal Antibody (Catalog # MAB5678) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005) or the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Mouse Reg3D Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse pancreas

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reg3D

Reg3D (regenerating islet-derived III delta), also called INGAPrP (islet neogenesis-associated protein-related protein) is an ~20 kDa member of the Reg family of secreted calcium-dependent C-type lectins. Reg proteins are involved in various biological functions, including acting as a survival/growth factors. Reg3D mRNA is expressed mainly in pancreatic islets and the digestive system. The mature 149 amino acid Reg3D shares ~50 - 70% amino acid sequence identity with other mammalian Reg3 proteins.

Long Name

Regenerating Islet-derived III delta

Alternate Names

INGAPrP

Entrez Gene IDs

30053 (Mouse)

Gene Symbol

REG3D

Additional Reg3D Products

Product Documents for Mouse Reg3D Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Reg3D Antibody

For research use only

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