Mouse/Rat Nestin Alexa Fluor™ Plus 555-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2736AFP555

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FAB2736AFP555-100UG
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 307501

View all Nestin Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat Nestin

Specificity

Detects mouse and rat Nestin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nestin

Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

References

  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol. 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2001) Biochem. Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.

Alternate Names

NES

Entrez Gene IDs

10763 (Human); 18008 (Mouse); 25491 (Rat)

Gene Symbol

NES

UniProt

EDM00749

Additional Nestin Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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