Mouse/Rat BMP-15/GDF-9B Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5917U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse BMP-15/GDF-9B
Gln268-Arg392
Accession # Q9Z0L4
Gln268-Arg392
Accession # Q9Z0L4
Specificity
Detects mouse and rat BMP-15/GDF-9B in Western blots and recombinant mouse in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) BMP‑15 is observed and less than 1% cross‑reactivity with rhBMP-3 a
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: BMP-15/GDF-9B
BMP-15 (Bone morphogenetic protein 15; also GDF-9B) is a 24-26 kDa member of the TGF-beta superfamily of proteins. It is expressed by late primary follicle oocytes, where it promotes the transition of preantral granulosa cells to cumulus cells, and later the expansion of cumulus cells. Mouse BMP-15 proprecursor is a 50-55 kDa, 367 amino acid (aa) glycoprotein. It is proteolytically cleaved to generate a 40 kDa prosegment (aa 26-267) plus a 24 kDa, 124 aa mature region (aa 268-392) that may be phosphorylated (on Ser7 of the mature molecule) and/or glycosylated. Secreted BMP-15 does not occur as a mature homodimer, but it does exist as a mature monomer, an uncleaved proprecursor, or as a noncovalent heterodimer composed of a cleaved mature region and its prosegment. The heterodimer may also form an oligomer. The BMP-15 prosegment reportedly forms a noncovalent heterodimer with 20 kDa mature GDF-9. Mature mouse BMP-15 shares 70% and 91% aa identity with human and rat BMP-15, respectively.
Long Name
Bone Morphogenetic Protein 15
Alternate Names
BMP15, GDF-9B
Gene Symbol
BMP15
UniProt
Additional BMP-15/GDF-9B Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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