Immersion fixed paraffin-embedded sections of mouse kidney and lung
Scientific Data Examples for Mouse Podoplanin Antibody
Western Blot
Detection of Mouse Podoplanin by Western Blot.
Western Blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # MAB11608) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Podoplanin at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Immunohistochemistry
Detection of Podoplanin in Mouse Kidney.
Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # mab11608) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of podocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Detection of Podpalanin in Mouse Lung.
Podoplanin was detected in immersion fixed paraffin-embedded sections of mouse lung using Rat Anti-Mouse Podoplanin Monoclonal Antibody (Catalog # mab11608) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell membrane of alveoli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator
Background: Podoplanin
Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.
Item
Value
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
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