Mouse Patched 1/PTCH Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11789
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 1119147
Product Specifications
Immunogen
Synthetic Peptide
Specificity
Detects a synthetic peptide specific for Mouse Patched 1 around amino acid 260 in Direct ELISA.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Patched 1/PTCH Antibody
Immersion fixed paraffin-embedded sections of mouse embryo
Patched 1/PTCH was detected in immersion fixed paraffin-embedded sections of mouse embryo using Rat Anti-Mouse Patched 1/PTCH Monoclonal Antibody (Catalog # MAB11789) at 15 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the ventral neural tube. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Mouse Patched 1/PTCH Antibody
Application
Recommended Usage
Immunohistochemistry
1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse embryo
Sample: Immersion fixed paraffin-embedded sections of mouse embryo
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Patched 1/PTCH
Patched is a 170‑175 kDa member of the patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to Patched, Smoothened is released and initiates downstream signaling. Mouse Patched is a 12 transmembrane, 1434 amino acid (aa) glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for Patched has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse Patched shares 97% and 99% aa sequence identity with human and rat Patched, respectively.
Alternate Names
BCNS, HPE7, mes, NBCCS, ptc1, PTCH1
Gene Symbol
PTCH1
Additional Patched 1/PTCH Products
Product Documents for Mouse Patched 1/PTCH Antibody
Product Specific Notices for Mouse Patched 1/PTCH Antibody
For research use only
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