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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant mouse PACAP/ADCYAP1
Pro26-Leu175
Accession # O70176

Specificity

Detects mouse PACAP/ADCYAP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 12% cross‑reactivity with recombinant human PACAP is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse PACAP/ADCYAP1 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PACAP/ADCYAP1

PACAP (Pituitary adenylate cyclase activating polypeptide) is a 17 kDa (predicted) member of the VIP/secretin/glucagon/GHRH superfamily of peptides. It is expressed by anterior pituitary gonadotrophs, neurons in the CNS and PNS, spermatids, adrenal chromaffin cells, plus macrophages and lymphocytes. PACAP has multiple effects. On mast cells, it induces histamine release; on lymphocytes, PACAP promotes Th2 development; in bone, PACAP inhibits osteoclastogenesis; and in the pituitary, PACAP facilitates the release of LH (in females), GH and alpha‑MSH. Mouse PACAP proprecursor is 151 amino acids (aa) in length (aa 25‑175). It undergoes proteolytic processing to generate two C‑terminal amidated peptides termed PACAP-38 (aa 131‑168) and PACAP-27 (aa 131‑157). In general, PACAP-38 represents the dominant form. However, the ratio between the -38 and -27 forms differs in a cell-specific manner. Over aa 25‑175, mouse PACAP shares 95% and 81% aa identity with rat and human PACAP, respectively.

Long Name

Pituitary Adenylate Cyclase-activating Polypeptide

Alternate Names

ADCYAP1

Entrez Gene IDs

116 (Human); 11516 (Mouse); 24166 (Rat)

Gene Symbol

ADCYAP1

UniProt

Additional PACAP/ADCYAP1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse PACAP/ADCYAP1 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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