Mouse OSMR beta Antibody

Detection of Mouse OSMR beta by Western Blot

OSM down-regulates GLT-1 and GLAST expression in primary mouse cortical astrocytes. a Total mRNA purified from wild-type (C57BL/6J) mouse neonatal (P2) brain cortex as well as from cultured cortical astrocytes established from P2 brains was analyzed for expression of OSMR-beta and gp130 mRNA by reverse transcriptase PCR; GAPDH primers were used as loading control. b Cortical astrocyte cultures were treated without or with OSM (10 ng/mL) for 2, 4, 8, 12, and 24 h and analyzed for GLT-1 and GLAST mRNA levels (gene expression normalized to HPRT1) by real-time PCR. Data are normalized to untreated controls and presented as mean ± SEM; n = 3, **p < 0.01, ***p < 0.001; one-way ANOVA using Bonferroni correction. c Cortical astrocytic cultures were treated without or with OSM (10 ng/mL) for 3, 5, and 24 h and were analyzed for GLAST proteins by Western blot. Relative densitometric analysis of GLAST proteins is shown in the lower panel. Data are presented as percentage of each respective ratio between optical density value of GLAST band intensity and optical density value of the matched alpha-tubulin (which served as the loading control) band intensity; n = 3, **p < 0.01, ***p < 0.001; one-way ANOVA Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27287400), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse OSMR beta by Western Blot

EcoHIV induces OSM release and gp130 mRNA, but not OSMR-beta mRNA or protein, in cultured primary mouse microglia. a Shows real-time PCR analysis of HIV LTR mRNA in control and EcoHIV-infected (35,000 pg of p24, for 24 h) primary microglia. ****p < 0.0001, n = 3. b Shows ELISA analysis of secreted OSM proteins in culture supernatants collected from control and EcoHIV-infected primary microglia. *p < 0.05, n = 6. c Shows real-time PCR analysis of gp130 and OSMR-beta mRNA in control and EcoHIV-infected primary microglia. *p < 0.05, n = 3. d Shows Western blot analysis for OSMR-beta proteins in control and EcoHIV-infected primary microglial cell lysates obtained from three independent experiments. beta-Actin served as the loading control Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27287400), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse OSMR beta by Western Blot

EcoHIV neither induces OSM secretion, nor the expression of gp130 or GLAST mRNA, but stimulates the expression of GLT-1 and OSMR-beta in primary cortical astrocytes. a Shows real-time PCR analyses of HIV LTR mRNA in control and EcoHIV-infected (35,000 pg of p24, for 24 h) cortical astrocyte cultures. ****p < 0.0001, n = 3. b Shows ELISA analysis of secreted OSM proteins in culture supernatants collected from control and EcoHIV-infected primary astrocyte cultures. n = 6. c, d Shows real-time PCR analyses of GLAST and GLT-1 (c) and gp130 and OSMR-beta (d) mRNA in control and EcoHIV-infected cortical astrocyte cultures. *p < 0.05, **p < 0.01, n = 3. e Shows Western blot analyses for OSMR-beta proteins in control and EcoHIV-infected cortical astrocyte culture lysates. The left panel shows representative blot of three independent experiments. The right panel shows densitometric analysis of OSMR-beta proteins. Data are presented as percentage of each respective ratio between optical density value of OSMR-beta band (110 kDa) intensity and optical density value of the matched beta-actin (42 kDa; loading control) band intensity. *p = 0.027, n = 3; two-tailed Student’s t test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27287400), licensed under a CC-BY license. Not internally tested by R&D Systems.