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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 523808

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Nogo-B.
Met1-Ile183 (predicted)
Accession # Q99P72

Specificity

Detects mouse Nogo-B in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) Nogo-B and recombinant rat Nogo-A and no cross-reactivity with rhNogo-A is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse Nogo-B Alexa Fluor® 405-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nogo-B

Nogo-B, also known as ASY, is a 55 kDa splice form of Nogo-A/Reticulon-4 and is primarily expressed in vascular endothelial cells. Nogo-A contains a large N‑terminal cytoplasmic domain and a reticulon region that consists of two transmembrane segments with an intervening noncytoplasmic domain. Nogo-B lacks most of the N-terminal domain but retains the reticulon domain. It inhibits neointimal hyperplasia in the vasculature and is downregulated in atherosclerotic tissue and thoracic arterial aneurisms. Interaction of Nogo-B with NgBR promotes endothelial cell proliferation and migration. Within aa 1‑183, mouse Nogo-B shares 76% and 94% aa sequence identity with human and rat Nogo-B, respectively.

Long Name

Reticulon 4B

Alternate Names

NogoB, RTN4B

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Gene Symbol

RTN4

UniProt

Additional Nogo-B Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Nogo-B Alexa Fluor® 405-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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