Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB22252G) and either (A) Rat Anti-Mouse NKG2A/CD159a APC-conjugated Monoclonal Antibody (Catalog # FAB6867A) or (B) Rat IgG_2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse NKG2A/CD159a APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

0.25-1 µg/10⁶ cells
Sample: Mouse splenocytes

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a 244 amino acid (aa), ~38 kDa type II transmembrane glycoprotein with a cytoplasmic ITIM motif and a single extracellular lectin-like domain. Within the extracellular domain (aa 94-244), mouse NKG2A shares 71% and 41% aa sequence identity with rat and human NKG2A, respectively. An isoform lacking aa 98-114 has been described. NKG2A associates with CD94 and is coexpressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its murine ligand, Qa-1b (or HLA-E in humans) on antigen presenting cells.

Long Name

Natural Killer G2A

Alternate Names

CD159a, Klrc1, NKG2

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Gene Symbol

KLRC1

UniProt

Q9Z202

Additional NKG2A/CD159a Products

Product Documents for Mouse NKG2A/CD159a APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse NKG2A/CD159a APC-conjugated Antibody

For research use only

Citations
Reviews

Mouse NKG2A/CD159a APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6867A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all NKG2A/CD159a Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse NKG2A/CD159a APC-conjugated Antibody

Detection of NKG2A/CD159a in Mouse Splenocytes by Flow Cytometry.

Applications for Mouse NKG2A/CD159a APC-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: NKG2A/CD159a

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional NKG2A/CD159a Products

Product Documents for Mouse NKG2A/CD159a APC-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Mouse NKG2A/CD159a APC-conjugated Antibody

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