Detects mouse Neutrophil Elastase/ELA2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human ELA2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Neutrophil Elastase/ELA2 Antibody

Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot.

Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse Neutrophil Elastase/ELA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neutrophil Elastase/ELA2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Neutrophil Elastase/ELA2 Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Mouse bone marrow

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as Leukocyte Elastase, Bone marrow serine protease, Medullasin and ELANE, is a 25-30 kDa serine protease belonging to the peptidase S1 family. ELA2 is secreted by neutrophils during inflammation, it destroys bacteria and host tissue. ELA2 function is the degradation of many extracellular matrix macromolecules. ELA2 is localized to keratohyalin granules, where it was found to directly participate in pro-filaggrin processing. When expressed aberrantly, ELA2 can cause emphysema or emphysematous changes, it involves breakdown of the lung structure and increased airspaces. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Entrez Gene IDs

1991 (Human); 50701 (Mouse)

Gene Symbol

ELANE

UniProt

Q3UP87

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Mouse Neutrophil Elastase/ELA2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Neutrophil Elastase/ELA2 Antibody

For research use only

Citations
Reviews

Mouse Neutrophil Elastase/ELA2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4517

Key Product Details

Species Reactivity

Validated:

Cited:

Applications