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Mouse Nanog Alexa Fluor™ Plus 555-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2729AFP555

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AF2729AFP555-100UG

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Specificity

Detects recombinant mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Nanog Alexa Fluor™ Plus 555-conjugated Antibody

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency
(1-3).

References

  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Entrez Gene IDs

79923 (Human); 71950 (Mouse)

Gene Symbol

NANOG

UniProt

Additional Nanog Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Nanog Alexa Fluor™ Plus 555-conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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