Detects mouse Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant human MEP1A is observed and less than 5% cross‑reactivity with recombinant mouse MEP1B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Meprin alpha Subunit/MEP1A Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Meprin alpha Subunit/MEP1A (Catalog # 4007-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Meprin alpha Subunit/MEP1A (Catalog # 4007-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meprin alpha Subunit/MEP1A

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or hetero-oligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 760 amino acid sequence of mouse meprin alpha subunit precursor consists of a signal peptide (residues 1‑33), a pro region (residues 34‑77), and a mature chain (residues 78‑760) containing the following domains, catalytic (residues 78‑275), MAM (residues 276‑445), MATH (residues 447‑607), EGF-like (residues 684‑724), transmembrane (residues 727‑754), and cytoplasmic (residues 755‑760) (5). The pro enzyme terminating at residue 615 was expressed and the secreted protein purified from conditioned medium. The molecular masses of recombinant mouse MEP1A are similar to those observed for the alpha subunit of rat Meprin A (6).

References

Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
Stocker, W. et al. (1995) Protein Sci. 4:823.
Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.
Jiang, W. et al. (1992) J. Biol. Chem. 267:9185.
Bertenshaw, G.P. et al. (2003) J. Biol. Chem. 278:2522.

Alternate Names

MEP1A, PPHA

Entrez Gene IDs

4224 (Human); 17287 (Mouse); 25684 (Rat)

Gene Symbol

MEP1A

UniProt

NP_032611

Additional Meprin alpha Subunit/MEP1A Products

Product Documents for Mouse Meprin alpha Subunit/MEP1A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Meprin alpha Subunit/MEP1A Antibody

For research use only

Citations
Reviews

Mouse Meprin alpha Subunit/MEP1A Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4007

Key Product Details

Species Reactivity

Validated:

Cited:

Applications