Mouse splenocytes were stained with either Rat Anti-Mouse Integrin alpha 5/CD49e (MAB1754, closed histogram) or Rat IgG2B Isotype Control (MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin alpha5/CD49e Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Mouse Splenocytes

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed L-929 mouse fibroblast cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Integrin alpha5/CD49e

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

Integrin alpha5 subunit, also called CD49e, associates with the Integrin beta1 subunit (CD29) to form the VLA-5 complex. It is expressed on thymocytes, mast cells, activated T lymphocytes, and some mouse cell lines. It functions as a receptor for fibronectin (1‑4).

References

Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Kinashi, T. and T.A. Springer (1994) Blood Cells 20:25.
Halvorson, M.J. et al. (1995) J. Immunol. 55:4567.
Uhlenkott, E.C. et al. (1996) Clin. Exp. Metastasis 14:125.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

P11688

Additional Integrin alpha 5/CD49e Products

Product Documents for Mouse Integrin alpha5/CD49e Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Integrin alpha5/CD49e Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry