Recombinant Mouse IL-22 stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 Secretion elicited by Recombinant Mouse IL-22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB5821). The ND₅₀ is typically 0.5-6 µg/mL.

Applications for Mouse IL-22 Antibody

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize IL‑22-induced IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.5-6 µg/mL in the presence of 500 pg/mL Recombinant Mouse IL‑22.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. The mouse IL-22 gene is localized to chromosome 10. Although it exists as a single copy gene in many mouse strains, the IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References

Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
Dumoutier, L. et al. (2000) PNAS 97:10144.
Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Long Name

Interleukin 22

Alternate Names

IL-TIF, IL22

Entrez Gene IDs

50616 (Human); 50929 (Mouse); 500836 (Rat)

Gene Symbol

IL22

UniProt

Q9JJY9

Additional IL-22 Products

Product Documents for Mouse IL-22 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IL-22 Antibody

For research use only

Citations
Reviews

Mouse IL-22 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5821

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Label

Antibody Source

View all IL-22 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Mouse IL-22 Antibody

IL-10 Secretion Induced by IL-22 and Neutralization by Mouse IL-22 Antibody.