Mouse IL-21 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF594
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
Flow Cytometry, ELISA Detection, ELISA Development, ELISpot Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
Pro25-Ser146
Accession # Q9ES17
Pro25-Ser146
Accession # Q9ES17
Specificity
Detects mouse IL-21 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL‑15, and recombinant human IL‑21 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse IL-21 Biotinylated Antibody
Mouse IL-21 ELISA Standard Curve
Recombinant Mouse IL-21 (Catalog # 594-ML) was serially diluted and captured by Goat Anti-Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse IL-21 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL-21 (Catalog # 594-ML)
Sample: Recombinant Mouse IL-21 (Catalog # 594-ML)
Mouse IL-21 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-21
Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gammac) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.
References
- Parrish-Novak, et al. (2000) Nature 408:57.
- Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
- Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
- Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21
Alternate Names
CVID11, IL21, Za11
Gene Symbol
IL21
UniProt
Additional IL-21 Products
Product Documents for Mouse IL-21 Biotinylated Antibody
Product Specific Notices for Mouse IL-21 Biotinylated Antibody
For research use only
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