Mouse FGFR2 (IIIb) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7161
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 133730
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FGF R2 isoforms and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse FGF R2 isoforms
Specificity
Detects IIIb isoforms of mouse FGF R2 in direct ELISAs and Western blots. This antibody shows approximately 75% cross-reactivity with recombinant human (rh) FGF R2 (IIIb) isoforms and no cross-reactivity with the IIIc isoform of mouse or human FGF R2 or any isoforms of rhFGF R1, rmFGF R3, or rhFGF R4.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse FGFR2 (IIIb) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse FGF R2 alpha (IIIb) Fc Chimera (Catalog # 5496-MF)
Recombinant Mouse FGF R2 beta (IIIb) Fc Chimera (Catalog # 708-MF)
under non-reducing conditions only
Sample: Recombinant Mouse FGF R2 alpha (IIIb) Fc Chimera (Catalog # 5496-MF)
Recombinant Mouse FGF R2 beta (IIIb) Fc Chimera (Catalog # 708-MF)
under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGFR2
FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.
Long Name
Fibroblast Growth Factor Receptor 2
Alternate Names
CD332, FGF R2
Gene Symbol
FGFR2
Additional FGFR2 Products
Product Documents for Mouse FGFR2 (IIIb) Antibody
Product Specific Notices for Mouse FGFR2 (IIIb) Antibody
For research use only
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