Mouse EMMPRIN/CD147 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF772
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Western Blot, Flow Cytometry
Cited:
Immunohistochemistry, Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
Ala22-Arg209
Accession # NP_001070652
Ala22-Arg209
Accession # NP_001070652
Specificity
Detects mouse EMMPRIN/CD147 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human EMMPRIN is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse EMMPRIN/CD147 Biotinylated Antibody
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Flow Cytometry
Flow cytometric analysis reveals proper dual-expression of both mCD147 and hCD147 in PBMCs and various organs. Representative contour plots of CD147 expression on WT-NSG (top) and hCD147KIhet-NSG (bottom) cells from (A) PBMCs, (B) lung, (C) liver, and (D) spleen using antibodies targeting either mouse CD147 protein, human CD147 protein, or a combination of both antibodies (far right). Relative percentages are listed, and significant shifts highlighted in red. Gating was determined based on donkey anti-goat/mouse isotype IgG antibody background staining Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35690792), licensed under a CC-BY license. Not internally tested by R&D Systems.
Applications for Mouse EMMPRIN/CD147 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse thymocytes
Sample: Mouse thymocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse EMMPRIN/CD147 Fc Chimera
Sample: Recombinant Mouse EMMPRIN/CD147 Fc Chimera
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EMMPRIN/CD147
EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.
Long Name
Extracellular Matrix Metalloproteinase Inducer
Alternate Names
Basigin, BSG, CD147
Gene Symbol
BSG
UniProt
Additional EMMPRIN/CD147 Products
Product Documents for Mouse EMMPRIN/CD147 Biotinylated Antibody
Product Specific Notices for Mouse EMMPRIN/CD147 Biotinylated Antibody
For research use only
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