Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Chk2 Monoclonal Antibody (Catalog # MAB22851) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Chk2 at approximately 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Chk2 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver and lung

Western Blot

2 µg/mL
Sample: Balb/3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line, under reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chk2

Checkpoint kinase 2 (Chk2) is a critical protein kinase involved in the cellular response to DNA damage. Chk2 is a target of the ATM protein kinase, and can phoshorylate multiple proteins, including Cdc25A and p53, to activate the G₁/S cell cycle checkpoint. Patients with Li-Fraumeni syndrome, a cancer-prone familial disorder, may have a mutation in the chk2 or p53 genes, which suggests that Chk2 also functions as a tumor suppressor.

Long Name

Checkpoint Kinase 2

Alternate Names

CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53

Entrez Gene IDs

11200 (Human); 50883 (Mouse); 114212 (Rat)

Gene Symbol

CHEK2

UniProt

Q9Z265

Additional Chk2 Products

Product Documents for Mouse Chk2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Chk2 Antibody

For research use only

Citations
Reviews

Mouse Chk2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB22851

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Chk2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Chk2 Antibody

Detection of Mouse Chk2 by Western Blot.