HEK293 human embryonic kidney cell line transfected with either (A) mouse CEACAM-2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse CEACAM-2 Monoclonal Antibody (Catalog # MAB9214) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CEACAM-2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CEACAM-2 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-2

CEACAM2 is a member of the family of carcinoembryonic antigen-related cell adhesion molecules. CEACAM2 controls energy balance and peripheral insulin action. It is involved in the regulation of feeding behavior particularly in the ventromedial nucleus of hypothalamus (VMH) regulation of food intake. Alternatively spliced transcripts encoding different proteins have been described.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 2

Alternate Names

AI267129, Bgp2, CEACAM2, Ceacam2-L

Entrez Gene IDs

26367 (Mouse)

Gene Symbol

CEACAM2

UniProt

Q925P2

Additional CEACAM-2 Products

Product Documents for Mouse CEACAM-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CEACAM-2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CEACAM-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9214

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CEACAM-2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse CEACAM-2 Antibody

Detection of CEACAM-2 in HEK293 Human Cell Line Transfected with Mouse CEACAM-2 and eGFP by Flow Cytometry.