Mouse CD5 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB115

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Complement-dependent Cytotoxicity, Blocking B Cell Proliferation, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 53-7.3

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Product Specifications

Immunogen

Mouse thymus or spleen

Specificity

Detects mouse CD5.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse CD5 Antibody

Application
Recommended Usage

Blocking B Cell Proliferation

Bikah, G. et al. (1998) Int. Immunol. 10:1185.

Complement-dependent Cytotoxicity

Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.

CyTOF-reported

Spitzer, M. et al. (2015) Science 349: 1259425. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Immunohistochemistry

van Ewijk, W. et al. (1981) J. Immunol. 127:2594.

Immunoprecipitation

Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.

References

  1. Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
  4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
  5. Luo, W. et al. (1992) J. Immunol. 148:1630.
  6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.

Alternate Names

CD5

Entrez Gene IDs

921 (Human); 12507 (Mouse)

Gene Symbol

CD5

Additional CD5 Products

Product Documents for Mouse CD5 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD5 Antibody

For research use only

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