Mouse CD24 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB8547P
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Recombinant Monoclonal Rat IgG2A Clone # 910019
Product Specifications
Immunogen
Peptide sequence spanning a portion of the extracellular region
Accession # P24807
Accession # P24807
Specificity
Detects mouse CD24a in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD24 PE-conjugated Antibody
Detection of CD24 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD24 PE-conjugated Monoclonal Antibody (Catalog # FAB8547P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Detection of CD24 by Flow Cytometry
4SC-202 reduces ALDHhigh and CD44high/CD24low cell populations. (a) 4T1 cells were treated with 4SC-202 at the indicated concentrations in the 3D scaffolds and analyzed by flow cytometry for a CD44high/CD24low cancer stem cell (CSC) profile. (b) Contour plot representing gating strategy, and (c) quantification of CD44high/CD24low CSC population normalized to beads. Significance is tested using ANOVA followed by a Dunnett’s test for n = 3 scaffold experiments (p < 0.001 ***). (d) Histogram plot revealing loss of CD24 expression with increased treatment. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35406526), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse CD24 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD24
CD24, also known as Heat Stable Antigen (HSA), is a heavily and variably glycosylated 35-60 kDa GPI-linked sialoprotein. CD24 is expressed on B cells, T cells, keratinocytes, and myofiber synaptic nuclei and is upregulated in a wide variety of cancers. It is also a marker for exosomes released into the urine and amniotic fluid. CD24 binds to P-Selectin on activated platelets and vascular endothelial cells. CD24 ligation induces tumor cell apoptosis and is important for the deletion of autoreactive T cells. Mature mouse CD24 shares 30% aa sequence identity with human CD24.
Alternate Names
CD24, CD24A
Gene Symbol
CD24
UniProt
Additional CD24 Products
Product Documents for Mouse CD24 PE-conjugated Antibody
Product Specific Notices for Mouse CD24 PE-conjugated Antibody
For research use only
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