Mouse CD11b/Integrin alpha M Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1124F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry
Cited:
Immunohistochemistry, Flow Cytometry, Cell Culture
Label
FITC (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # M1/70
Product Specifications
Immunogen
Con A-activated C57BL/10 splenocytes
Specificity
Detects mouse Integrin alphaM/CD11b. Cross-reaction with human Integrin alphaM has been reported (1, 2).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CD11b/Integrin alpha M Fluorescein-conjugated Antibody
Detection of Integrin alphaM/CD11b in Mouse Peripheral Blood Cells by Flow Cytometry.
Mouse peripheral blood cells were stained with Rat Anti-Mouse Integrin aM/CD11b Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1124F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Integrin alphaM/CD11b in Mouse Bone Marrow Cells by Flow Cytometry.
Mouse bone marrow cells were stained with (A) Rat Anti-Mouse Integrin aM/CD11b Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1124F) or (B) isotype control antibody (IC013F) and Rat anti-Mouse Gr-1/Ly-6G APC-conjugated Antibody (FAB1037A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD11b/Integrin alpha M Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse bone marrow cells and peripheral blood cells
Sample: Mouse bone marrow cells and peripheral blood cells
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alphaM subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta2 subunit (CD18) to form the non-covalent heterodimer Integrin alphaM/ beta2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alphaM/ beta2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alphaM/ beta2 can bind several ligands (including ICAM-1, Fibrinogen, and the C3 Complement Fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
Additional CD11b/Integrin alpha M Products
Product Documents for Mouse CD11b/Integrin alpha M Fluorescein-conjugated Antibody
Product Specific Notices for Mouse CD11b/Integrin alpha M Fluorescein-conjugated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...