Detects mouse BMP-5 in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human BMP-5 is observed. In direct ELISAs, approximately 100% cross-reactivity with recombinant human BMP-5 is observed, and approximately 4% cross-reactivity with recombinant mouse (rm) BMP-6 and rmBMP-7 is observed, and less than 1% cross-reactivity with rmBMP-8b is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse BMP-5 Antibody

Detection of Mouse BMP‑5 by Western Blot.

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMP-5 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BMP‑5 in Mouse Embryo.

BMP-5 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6176) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse BMP-5 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Western Blot

1 µg/mL
Sample: Mouse small intestine tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-5

Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta) superfamily (1). BMP-5 is synthesized as a 452 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2, 3). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature mouse BMP-5 shares 96% and 99% aa sequence identity with human and rat BMP-5, respectively. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (4). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (4, 5). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the short ear phenotype in mice (3, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is upregulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).

References

Chen, D. et al. (2004) Growth Factors 22:233.
Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
King, J.A. et al. (1994) Dev. Biol. 166:112.
Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
Jin, Y. et al. (2001) Oral Oncol. 37:225.
Ro, T.B. et al. (2004) Oncogene 23:3024.

Long Name

Bone Morphogenetic Protein 5

Alternate Names

BMP5

Entrez Gene IDs

653 (Human); 12160 (Mouse)

Gene Symbol

BMP5

UniProt

P49003

Additional BMP-5 Products

Product Documents for Mouse BMP-5 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse BMP-5 Antibody

For research use only

Citations
Reviews

Mouse BMP-5 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6176

Key Product Details

Species Reactivity

Validated:

Cited:

Applications