Mouse BMP-4 Antibody

Detection of Mouse BMP‑4 by Western Blot.

Western blot shows Recombinant Mouse BMP-4 (Catalog # 5020-BP) and lysates of mouse embryo tissue. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for BMP-4 at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BMP‑4 in Mouse Embryo.

BMP-4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) at 2 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastrointestinal tract cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of BMP-4 by Western Blot

BMPR1a deficiency resulted in reduced myoepithelial lineages and compromised repopulating capacity of mammary stem cells. (A) FACS analysis of mammary gland epithelial cells from control and cKO mice at pregnancy day 14.5 using the Lin/CD24/CD29/CD61 surface marker set. n = 4 mice per group. (B) Quantification of Lin– CD24+CD29high (Myo), Lin– CD24+CD29low (Lu) and Lin– CD24+CD29lowCD61+ cell populations in control and cKO mice at pregnancy day 14.5. n = 4 biological replicates. (C) Immunofluorescence staining for K14 (red) and K8 (green) in control and cKO mammary glands at pregnancy day 14.5. n = 3 mice per group. Scale bar, 25 μm. (D) Wstern blotting for K14 in control and cKO mammary glands at pregnancy day 14.5. beta-Actin was used as a loading control. Statistical analysis the expression of K14/ beta-Actin. n = 3 mice. (E) Immunofluorescence staining for K14 (red) and K8 (green) in HC11 mammary epithelial cells treated with BMP4 (50 ng/mL) for 24 h. n = 3 biological replicates. Scale bar, 25 μm. (F) Wstern blotting for K14 in HC11 cells treated with BMP4 (50 ng/mL) for 24 h. beta-Actin was used as a loading control. Statistical analysis the expression of K14/ beta-Actin. n = 4 biological replicates. (G) Illustration of the mammary transplantation assays using K14-rtTA/teto-Cre/Bmpr1afl/fl and control mouse mammary stem cells and the induction strategy. (H) Whole-mount staining of mammary glands from the mammary transplantation assays and quantification of the proportion of ductal trees. n = 5 mice per group. Scale bar, 1 mm. (I) Immunohistochemistry staining for Integrin beta3 in control and cKO mammary glands at pregnancy day 14.5. Quantification of Integrin beta3+ cells in alveoli. n = 3 mice per group. Scale bar, 50 μm. Data were presented as means ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34336839), licensed under a CC-BY license. Not internally tested by R&D Systems.