Mouse Bcl-2 Minus C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB810
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 121529
Product Specifications
Immunogen
E. coli-derived recombinant mouse Bcl-2
Specificity
Detects mouse Bcl‑2 Minus C-Terminus in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Bcl-2 Minus C-Terminus Antibody
Detection of Mouse Bcl-2 by Western Blot.
Western blot shows lysates of CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse Bcl-2 Minus C-Terminus Monoclonal Antibody (Catalog # MAB810) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Bcl-2 Minus C-Terminus (Catalog # 827-BC) (2 ng) was included. A specific band for Bcl-2 was detected at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Rat Bcl-2 by Simple Western
TUNEL assay of cardiac myocyte apoptosis in the hearts. (A) Quantification of TUNEL-positive nuclei n=3. Representative qPCR (B) and western blot (C) analysis (original blots/gels are presented in Supplementary 2 Fig.7-9) of apoptosis-related mRNA and protein, n=3, Chemiluminescence signals were visualized and cropped using Protein Simple Compass software. The whole cell lysates were used for western blot. The data are presented as the mean SEM; *P<0.05; **P<0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/40188200/), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Mouse Bcl-2 Minus C-Terminus Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: CTLL-2 mouse cytotoxic T cell line
Sample: CTLL-2 mouse cytotoxic T cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-2
Long Name
B Cell Lymphoma/Leukemia 2
Alternate Names
Bcl2
Gene Symbol
BCL2
Additional Bcl-2 Products
Product Documents for Mouse Bcl-2 Minus C-Terminus Antibody
Product Specific Notices for Mouse Bcl-2 Minus C-Terminus Antibody
For research use only
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