Mouse B7-H3 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB10271AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2741E
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived mouse B7-H3
Specificity
Detects mouse B7-H3 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Applications for Mouse B7-H3 Alexa Fluor™ Plus 405-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: B7-H3
T cells require a signal induced by the engagement of the T cell receptor and a “costimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with costimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (1). B7-H3 is expressed at very high levels in immature dendritic cells at moderate levels on mature dendritic cells, LPS stimulated immature dendritic cells and LPS stimulated monocytes, and at low levels on resting monocytes. B7-H3 binds to activated T cells via an as-of-yet identified receptor. B7-H3 co-stimulates proliferation of T cells and interferon-gamma (IFN-gamma) production and enhances the induction of cytotoxic T cells. B7-H3 shares 20 - 27% amino acid (aa) identity with other B7 family members (2). Murine B7-H3 is a 259 aa protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Mouse and human B7-H3 share 87% aa identity (3).
References
- Coyle, A.J. and J.-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
- Chapoval, A.I. et al. (2001) Nature Immunol. 2:269.
- Sun, M. et al. (2002) J. Immunol. 168:6294.
Long Name
B7 Homolog 3
Alternate Names
B7H3, CD276
Gene Symbol
CD276
UniProt
Additional B7-H3 Products
Product Specific Notices for Mouse B7-H3 Alexa Fluor™ Plus 405-conjugated Antibody
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
Recommended applications are based on validated applications from the unconjugated base product MAB10271.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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