Mouse B220/CD45R Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1217
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-reported
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # RA3-6B2
Product Specifications
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Specificity
Detects mouse B220/CD45R.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse B220/CD45R Antibody
B220/CD45R in Mouse Splenocytes.
B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Monoclonal Antibody (Catalog # MAB1217) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Detection of B220/CD45R by Flow Cytometry
pDCs downregulate Siglec-H upon MCMV infection in a TLR9- and MyD88-dependent manner in vitro.Flt3-L derived total BMDCs or CD11c+B220+ pDCs sorted from BMDC cultures were mock treated with PBS, CpG treated or MCMV infected at MOI 2 unless indicated otherwise. (A) Representative FACS plots show Siglec-H expression and MCMV-GFP expression of BMDCs (upper panel). Representative histogram plots display B220 expression on CD11c+ Siglec-H+ DCs at 0, 3, 6 and 24 h p.i. compared with mock treated controls (lower panel). (B) Representative histogram plots display Siglec-H expression of sorted wt, TLR9−/− and MyD88−/− pDCs after stimulation or infection. (C) Representative histogram plots display Siglec-H expression of sorted wt and IFNAR−/− pDCs 24 h after infection or stimulation. (D) Quantification of the Siglec-H expression from wt and IFNAR−/− pDCs after MCMV infection relative to their respective mock controls. Data are from one of three individual experiments with similar results. *p<0.05, ***p<0.001, Students t-test. (Gray bar = isotype control, gray dashed line = mock treatment and dark bold line = CpG-A treatment or MCMV infection). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24086137), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse B220/CD45R Antibody
Application
Recommended Usage
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Immunoprecipitation
Coffman, R.L. (1982) Immuno. Rev. 69:5.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B220/CD45R
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
References
- Coffman, R.L. (1982) Immuno. Rev. 69:5.
- Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
- Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
- Allman, D.M. et al. (1992) J. Immunol. 149:2533.
- Asensi, V.K. et al. (1989) Immunology 68:204.
- Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name
Cluster of Differentiation 45
Alternate Names
CD45R
Gene Symbol
PTPRC
Additional B220/CD45R Products
Product Documents for Mouse B220/CD45R Antibody
Product Specific Notices for Mouse B220/CD45R Antibody
For research use only
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