Mouse ALCAM/CD166 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1173N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry, Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 200622
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166
Trp28-Lys527
Accession # AAC06342
Trp28-Lys527
Accession # AAC06342
Specificity
Detects mouse ALCAM/CD166 in Western blots and direct ELISAs. In Western blots, shows 100% cross-reactivity with recombinant human (rh) ALCAM and no cross-reactivity with rhBCAM, rhEpCAM, recombinant mouse (rm) MAdCAM-1, rhMCAM, rmNCAM-L1, rmOCAM, or rmTROP-2.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse ALCAM/CD166 Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Mouse splenocytes treated with PHA
Sample: Mouse splenocytes treated with PHA
Western Blot
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ICAM-5
Intercellular adhesion molecule-5 (ICAM-5), also known as telencephalin, is an integral membrane glycoprotein expressed in neurons of mammalian telencephalons. ICAM-5 is a member of the immunoglobulin superfamily and shares 38-55% amino acid homology with other ICAMs. Structurally, ICAM-5 contains nine Ig domains that included 15 N-glycosylation sites, a single transmembrane region, and C-terminal cytoplasmic tail (1). As with other members of the ICAM family, ICAM-5 has been shown to be involved in cellular adhesion. ICAM-5 binds to the leukocyte integrin LFA-1 (CD11a/CD18) via its first NH2-terminal Ig domain. The ability of ICAM-5 to bind LFA-1 suggests that ICAM-5 may play an important role in immune responses in the central nervous system (2). Additionally, ICAM-5 has been found to promote homophilic binding via binding of the first Ig domain to Ig domains 4-5. Homophilic adhesion activity of ICAM-5 is regulated by a monomer/tetramer transition. ICAM-5 expression temporally parallels the onset of dendritic elongation and synaptogenesis during the postnatal period suggesting that ICAM-5 may provide a brain segment-specific cue for synaptogenesis or dendrite-dendrite interaction (3).
References
- Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
- Aruffo, A. et al. (1997) Immunol. Today 18:498.
- Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
- Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.
Long Name
Intercellular Adhesion Molecule 5
Alternate Names
ICAM5, Telencephalin
Gene Symbol
ICAM5
UniProt
Additional ICAM-5 Products
Product Specific Notices for Mouse ALCAM/CD166 Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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