MMP-9 Antibody (S51-82) - BSA Free

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699]

MMP-9-Antibody-S51-82-Immunocytochemistry-Immunofluorescence-NBP2-59699-img0003.jpg

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (S51-82) - BSA Free [NBP2-59699] -

Identification of MET-like structures within human fetal membrane tissues infected with GBS ex vivo. Fetal membrane tissues were excised from healthy, term placental tissues from women undergoing routine cesarean section. (A and B) Fetal membrane tissues were then infected with GBS on the choriodecidual surface for 48 h prior to fixation and immunohistochemistry (A) or confocal microscopy (B). (A) Fetal membrane tissues were stained with hematoxylin and eosin (H & E), and representative images are shown at a magnification of ×4. The area within the red box is shown in sections stained with anti-GBS, anti-CD163, or anti-histone H3 antibodies and visualized by immunohistochemistry. GBS cells are able to invade from the choriodecidual surface (CD) toward the amnion epithelium (AE). Macrophages are shown in the area of GBS infection, and macrophages with extracellular histone staining (rightmost image, insert with ×40 magnification) are demonstrated in an area that is also stained with the macrophage marker CD163 (red boxes). Bars represent 100 μm. (B) Fixed and paraffin-embedded fetal membranes were stained with conjugated primary antibodies against CD163, histone H3, and MMP-9. CD163-positive cells within the membrane tissue are seen extruding contents that stain positive for histones and MMP-9 consistent with METs (white arrowheads). Bars represent 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30459195), licensed under a CC-BY license. Not internally tested by Novus Biologicals.